饮用水100毫升样本中大肠杆菌噬菌体的实用直接噬菌斑测定法。
Practical direct plaque assay for coliphages in 100-ml samples of drinking water.
作者信息
Grabow W O, Coubrough P
出版信息
Appl Environ Microbiol. 1986 Sep;52(3):430-3. doi: 10.1128/aem.52.3.430-433.1986.
Abstract
A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.
摘要
设计并评估了一种用于直接检测100毫升水样中大肠杆菌噬菌体的实用单琼脂层噬菌斑检测法。使用该检测法时，将100毫升水样、含有二价阳离子的琼脂培养基和宿主大肠杆菌C（ATCC 13706）在一个容器中混合，然后将混合物铺在10个直径为14厘米的培养皿上。该方法比其他各种方法更灵敏、可靠和准确，且证明快速、简单且经济。
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