饮用水100毫升样本中大肠杆菌噬菌体的实用直接噬菌斑测定法。
Practical direct plaque assay for coliphages in 100-ml samples of drinking water.
作者信息
Grabow W O, Coubrough P
出版信息
Appl Environ Microbiol. 1986 Sep;52(3):430-3. doi: 10.1128/aem.52.3.430-433.1986.
Abstract
A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.
摘要
设计并评估了一种用于直接检测100毫升水样中大肠杆菌噬菌体的实用单琼脂层噬菌斑检测法。使用该检测法时，将100毫升水样、含有二价阳离子的琼脂培养基和宿主大肠杆菌C（ATCC 13706）在一个容器中混合，然后将混合物铺在10个直径为14厘米的培养皿上。该方法比其他各种方法更灵敏、可靠和准确，且证明快速、简单且经济。
相似文献
1
Practical direct plaque assay for coliphages in 100-ml samples of drinking water.饮用水100毫升样本中大肠杆菌噬菌体的实用直接噬菌斑测定法。
Appl Environ Microbiol. 1986 Sep;52(3):430-3. doi: 10.1128/aem.52.3.430-433.1986.
2
Comparison of selective media for assay of coliphages in sewage effluent and lake water.用于污水和湖水样本中大肠杆菌噬菌体测定的选择性培养基比较
Appl Environ Microbiol. 1985 Jan;49(1):33-6. doi: 10.1128/aem.49.1.33-36.1985.
3
A simple, rapid and sensitive presence/absence detection test for bacteriophage in drinking water.一种用于检测饮用水中噬菌体的简单、快速且灵敏的有无检测试验。
J Appl Bacteriol. 1993 Apr;74(4):490-6. doi: 10.1111/j.1365-2672.1993.tb05159.x.
4
A simplified method for coliphage detection in natural waters.
Acta Microbiol Pol. 1983;32(2):197-206.
5
Aptness of Escherichia coli host strain CB390 to detect total coliphages in Colombia.哥伦比亚用大肠杆菌宿主菌株 CB390 检测总大肠噬菌体的适宜性。
Sci Rep. 2019 Jun 25;9(1):9246. doi: 10.1038/s41598-019-45775-y.
6
Improved method for coliphage detection based on beta-galactosidase induction.基于β-半乳糖苷酶诱导的大肠杆菌噬菌体检测改进方法。
J Virol Methods. 1992 Oct;40(1):31-6. doi: 10.1016/0166-0934(92)90004-w.
7
A direct membrane filter method for enumerating somatic coliphages in drinking water.一种用于计数饮用水中体细胞噬菌体的直接膜过滤法。
Microbiologia. 1994 Sep;10(3):285-96.
8
Evaluation of Escherichia coli host strain CB390 for simultaneous detection of somatic and F-specific coliphages.评估大肠杆菌宿主菌株CB390用于同时检测体细胞噬菌体和F特异性噬菌体。
Appl Environ Microbiol. 2008 Jan;74(2):531-4. doi: 10.1128/AEM.01710-07. Epub 2007 Nov 16.
9
Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters.影响污水及受污水污染水体中大肠杆菌噬菌体计数的因素。
Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):387-97. doi: 10.1007/BF00399318.
10
Detection of infectious enteroviruses, enterovirus genomes, somatic coliphages, and Bacteroides fragilis phages in treated wastewater.处理后废水中传染性肠道病毒、肠道病毒基因组、体细胞噬菌体和脆弱拟杆菌噬菌体的检测
Appl Environ Microbiol. 1998 Nov;64(11):4307-12. doi: 10.1128/AEM.64.11.4307-4312.1998.
引用本文的文献
1
Synergistic effect of electrooxidation and anaerobic digestion of waste-activated sludge for microbial inactivation.废活性污泥电氧化与厌氧消化协同作用对微生物的灭活效果
Water Sci Technol. 2025 Feb;91(3):311-320. doi: 10.2166/wst.2025.016. Epub 2025 Feb 5.
2
An Appraisal of Bacteriophage Isolation Techniques from Environment.环境中噬菌体分离技术的评估
Microb Ecol. 2022 Apr;83(3):519-535. doi: 10.1007/s00248-021-01782-z. Epub 2021 Jun 17.
3
Genome rearrangements induce biofilm formation in Escherichia coli C - an old model organism with a new application in biofilm research.基因组重排诱导大肠杆菌 C 形成生物膜——一种在生物膜研究中具有新应用的古老模式生物。
BMC Genomics. 2019 Oct 22;20(1):767. doi: 10.1186/s12864-019-6165-4.
4
Assessment of recommended approaches for containment and safe handling of human excreta in emergency settings.评估在紧急情况下控制和安全处理人类排泄物的推荐方法。
PLoS One. 2018 Jul 26;13(7):e0201344. doi: 10.1371/journal.pone.0201344. eCollection 2018.
5
Complete Genome Sequence of Strain WG5.菌株WG5的全基因组序列
Genome Announc. 2018 Jan 11;6(2):e01403-17. doi: 10.1128/genomeA.01403-17.
6
Draft Genome Sequence of Bacteriophage vB_Eco_swan01.噬菌体vB_Eco_swan01的基因组序列草图
Genome Announc. 2017 Jul 13;5(28):e00501-17. doi: 10.1128/genomeA.00501-17.
7
Criteria for selection of surrogates used to study the fate and control of pathogens in the environment.用于研究环境中病原体的命运和控制的替代物选择标准。
Appl Environ Microbiol. 2012 Mar;78(6):1969-77. doi: 10.1128/AEM.06582-11. Epub 2012 Jan 13.
8
Bacteriophage Administration Reduces the Concentration of Listeria monocytogenes in the Gastrointestinal Tract and Its Translocation to Spleen and Liver in Experimentally Infected Mice.噬菌体给药可降低实验性感染小鼠胃肠道中单核细胞增生李斯特菌的浓度及其向脾脏和肝脏的转移。
Int J Microbiol. 2010;2010:624234. doi: 10.1155/2010/624234. Epub 2010 Jun 24.
9
Surface plasmon resonance assay for real-time monitoring of somatic coliphages in wastewaters.用于实时监测废水中体细胞噬菌体的表面等离子体共振分析
Appl Environ Microbiol. 2008 Jul;74(13):4054-8. doi: 10.1128/AEM.02806-07. Epub 2008 May 9.
10
Multilocus characterization scheme for shiga toxin-encoding bacteriophages.产志贺毒素噬菌体的多位点特征分析方案
Appl Environ Microbiol. 2007 Dec;73(24):8032-40. doi: 10.1128/AEM.01278-07. Epub 2007 Oct 19.
本文引用的文献
1
Evaluation of coliphage detection as a rapid indicator of water quality.将大肠杆菌噬菌体检测作为水质快速指标的评估。
Appl Environ Microbiol. 1982 Feb;43(2):430-4. doi: 10.1128/aem.43.2.430-434.1982.
2
The isolation of bacteriophages from the environment.从环境中分离噬菌体。
J Appl Bacteriol. 1982 Aug;53(1):1-17. doi: 10.1111/j.1365-2672.1982.tb04729.x.
3
Bacteriophage concentration from water by filter chromatography.通过过滤色谱法从水中浓缩噬菌体。
J Virol Methods. 1983 Dec;7(5-6):297-304. doi: 10.1016/0166-0934(83)90082-4.
4
Concentration of coliphages from drinking water by Mg(OH)2 flocculation.通过氢氧化镁絮凝法从饮用水中浓缩大肠杆菌噬菌体
Naturwissenschaften. 1983 Dec;70(12):612-3. doi: 10.1007/BF00377405.
5
Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters.影响污水及受污水污染水体中大肠杆菌噬菌体计数的因素。
Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):387-97. doi: 10.1007/BF00399318.
6
Concentration of coliphage from water and sewage with charge-modified filter aid.使用电荷改性助滤剂从水和污水中浓缩大肠杆菌噬菌体。
Appl Environ Microbiol. 1983 Jan;45(1):232-7. doi: 10.1128/aem.45.1.232-237.1983.
7
Coliphages as ecological indicators of enteroviruses in various water systems.作为各种水系统中肠道病毒生态指标的大肠杆菌噬菌体
Bull World Health Organ. 1981;59(4):611-8.
8
A simplified method for coliphage detection in natural waters.
Acta Microbiol Pol. 1983;32(2):197-206.
9
Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method.使用最大可能数法估算少量大肠杆菌噬菌体
Appl Microbiol. 1966 Mar;14(2):141-4. doi: 10.1128/am.14.2.141-144.1966.
10
Rapid determination of the presence of enteric bacteria in water.水中肠道细菌存在情况的快速测定
Appl Microbiol. 1974 Mar;27(3):484-7. doi: 10.1128/am.27.3.484-487.1974.
Zotero 插件