Pan T M, Pan F H
Institute of Applied Chemistry, Chinese Culture University, Taipei, Taiwan.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1992 Aug;25(3):129-37.
Strain CCU-U2-N4-41 was derived from a wild strain of yeast CCU-087. This strain can produce large amounts of citric acid from salad oil. The optimal medium for citric acid production is one liter of medium containing salad oil 100 g, yeast extract 2.0 g, CH3COONH4 3.0 g, KH2PO4 5.0 g, MgSO4 500 ppm, 1 ppm each of MnSO(4).5 H2O and CuSO(4).2 H2O, pH: 7.0. The optimal culture conditions are: temperature: 28 degrees C; agitation: 110 rpm; 100 mL medium in 500-mL Hinton flask; 1.5% CaCO3 added after 12 hr incubation. With 96 hr cultivation 71.2 g/L of citric acid can be produced. Citric acid produced was compared with authentic citric acid by melting point determination, IR, NMR and Mass. From the spectra, it was found that they were identical.
菌株CCU-U2-N4-41源自酵母野生菌株CCU-087。该菌株能从色拉油中大量产柠檬酸。产柠檬酸的最佳培养基是一升含有100克色拉油、2.0克酵母提取物、3.0克CH3COONH4、5.0克KH2PO4、500 ppm MgSO4、1 ppm MnSO(4).5 H2O和1 ppm CuSO(4).2 H2O的培养基，pH值为7.0。最佳培养条件为：温度：28℃；搅拌：110转/分钟；500毫升Hinton烧瓶中装100毫升培养基；培养12小时后添加1.5% CaCO3。经过96小时培养，可产71.2克/升柠檬酸。通过熔点测定、红外光谱、核磁共振和质谱将所产柠檬酸与纯柠檬酸进行比较。从光谱图可知，二者相同。
