Cupello A, Rapallino M V, Mainardi P
Centro di Neurofisiologia Cerebrale, CNR, Genova, Italy.
Int J Neurosci. 1992 Jan;62(1-2):35-43. doi: 10.3109/00207459108999755.
The basal and GABA activated accumulation of labelled chloride in rat cerebral cortex microsacs has been studied as a function of incubation time. Basal accumulation has biphasic kinetics within 2 minutes of incubation with two components clearly visible. GABA activated stimulation has two phases as well, starting from (GABA) = 10(-5) M, one appearing within seconds of incubation range and the other one within tens of seconds. Both GABA-activated components are blocked by bicuculline methiodide (BMI). Practically no effect by 10(-3) M nipecotic acid was found on either the comparatively rapid or the slower phase. The two GABA activated components may correspond to two different populations of sealed vesicles with different receptor concentration per internal volume.
研究了大鼠大脑皮质微囊中标记氯化物的基础积累以及GABA激活的积累与孵育时间的关系。基础积累在孵育2分钟内具有双相动力学，两个成分清晰可见。GABA激活的刺激也有两个阶段，从(GABA)=10(-5)M开始，一个在孵育范围内几秒内出现，另一个在几十秒内出现。两种GABA激活成分均被甲硫酸荷包牡丹碱（BMI）阻断。实际上，发现10(-3)M的尼克酸对较快或较慢阶段均无影响。两种GABA激活成分可能对应于两种不同的封闭囊泡群体，每个内部体积具有不同的受体浓度。
