文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Machado O L, Silva Júnior J G

Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.

Braz J Med Biol Res. 1992;25(6):567-82.

A 1.9S albumin having allergenic activity and denoted Ric c III was isolated from an alcohol extract of defatted Ricinus communis seeds, CB-1A, as a homogeneous protein by ion-exchange chromatography on SP-Sephadex, gel filtration on Sephadex G-75 and preparative polyacrylamide gel electrophoresis (6 mg Ric c III/g CB-1A). 2. The protein contained approximately 98 amino acid residues distributed in 2 chains of 67 and 34 residues, a molecular weight of 11,239 based on amino acid composition and pI = 4.9 Ric c III can be aligned, on the basis of amino acid composition and partial amino acid sequence data, with residues 18 to 50 (51) and 66 to 130 of the 2S albumin precursor predicted by the cDNA data of S.D. Irwin, J.N. Keen, J.B.C. Findlay and J.M. Lord (Molecular and General Genetics, 222: 400-408, 1990). 3. The present data identify Ric c III as the second allergenic 2S storage albumin coded by this DNA.

从脱脂蓖麻籽CB - 1A的醇提取物中，通过SP - Sephadex离子交换色谱、Sephadex G - 75凝胶过滤和制备性聚丙烯酰胺凝胶电泳，分离出一种具有致敏活性的1.9S白蛋白，命名为Ric c III，为一种纯蛋白（每克CB - 1A含6毫克Ric c III）。2. 该蛋白含有约98个氨基酸残基，分布在两条链上，分别为67个和34个残基，根据氨基酸组成计算分子量为11239，pI = 4.9。基于氨基酸组成和部分氨基酸序列数据，Ric c III可与S.D. Irwin、J.N. Keen、J.B.C. Findlay和J.M. Lord的cDNA数据预测的2S白蛋白前体的第18至50（51）位和66至130位残基比对（《分子与普通遗传学》，222: 400 - 408, 1990）。3. 目前的数据确定Ric c III为该DNA编码的第二种致敏性2S储存白蛋白。

Machado O L, Silva Júnior J G

Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.

Braz J Med Biol Res. 1992;25(6):567-82.

A 1.9S albumin having allergenic activity and denoted Ric c III was isolated from an alcohol extract of defatted Ricinus communis seeds, CB-1A, as a homogeneous protein by ion-exchange chromatography on SP-Sephadex, gel filtration on Sephadex G-75 and preparative polyacrylamide gel electrophoresis (6 mg Ric c III/g CB-1A). 2. The protein contained approximately 98 amino acid residues distributed in 2 chains of 67 and 34 residues, a molecular weight of 11,239 based on amino acid composition and pI = 4.9 Ric c III can be aligned, on the basis of amino acid composition and partial amino acid sequence data, with residues 18 to 50 (51) and 66 to 130 of the 2S albumin precursor predicted by the cDNA data of S.D. Irwin, J.N. Keen, J.B.C. Findlay and J.M. Lord (Molecular and General Genetics, 222: 400-408, 1990). 3. The present data identify Ric c III as the second allergenic 2S storage albumin coded by this DNA.

从脱脂蓖麻籽CB - 1A的醇提取物中，通过SP - Sephadex离子交换色谱、Sephadex G - 75凝胶过滤和制备性聚丙烯酰胺凝胶电泳，分离出一种具有致敏活性的1.9S白蛋白，命名为Ric c III，为一种纯蛋白（每克CB - 1A含6毫克Ric c III）。2. 该蛋白含有约98个氨基酸残基，分布在两条链上，分别为67个和34个残基，根据氨基酸组成计算分子量为11239，pI = 4.9。基于氨基酸组成和部分氨基酸序列数据，Ric c III可与S.D. Irwin、J.N. Keen、J.B.C. Findlay和J.M. Lord的cDNA数据预测的2S白蛋白前体的第18至50（51）位和66至130位残基比对（《分子与普通遗传学》，222: 400 - 408, 1990）。3. 目前的数据确定Ric c III为该DNA编码的第二种致敏性2S储存白蛋白。