ALLEN P Z, KABAT E A
J Exp Med. 1957 May 1;105(5):383-94. doi: 10.1084/jem.105.5.383.
Purified levan is antigenic in man and subcutaneous injection of 1 mg. leads to the production of precipitins and skin sensitivity. Apple amylopectin, maize glycogen, and synthetic polyglucose did not stimulate antibody production in small numbers of individuals injected. Quantitative precipitin studies carried out with several levan preparations employing human antilevan show variations in immunochemical behavior indicating structural differences among levans. Evidence that precipitins formed in response to levan injection are antilevan antibodies was obtained by analysis of specific precipitates formed in the region of antibody excess for ketosugar. Injection of levan giving rise to production of antilevan failed in one individual to give a non-specific anamnestic rise in either antidextran or antiblood group A precipitins. Laminarin, a neutral polysaccharide, was found to give precipitation with normal human sera.
纯化的左聚糖对人具有抗原性，皮下注射1毫克会导致沉淀素的产生和皮肤敏感性。苹果支链淀粉、玉米糖原和合成聚葡萄糖在少数注射的个体中不会刺激抗体产生。用几种左聚糖制剂与人抗左聚糖进行的定量沉淀素研究表明，免疫化学行为存在差异，这表明左聚糖之间存在结构差异。通过分析在抗体过量区域针对酮糖形成的特异性沉淀物，获得了注射左聚糖后形成的沉淀素是抗左聚糖抗体的证据。在一个个体中，注射左聚糖导致抗左聚糖的产生，但并未使抗右旋糖酐或抗血型A沉淀素出现非特异性回忆性升高。发现一种中性多糖海带多糖能与正常人血清发生沉淀反应。
