Smirnov I V, Khachatur'ian A A, Fetisova L V, Ivanov Iu G, Groznaia T G, Smirnova I L, Vorob'eva T M, Akimova T A, Iarochkin V S, Rozenberg G Ia
Gematol Transfuziol. 1992 Jul-Aug;37(7-8):15-7.
A study was made of bovine hemoglobin polymer obtained by a new method: intraerythrocytic polymerization of hemoglobin followed by isolation of hemoglobin polymer. The polymer obtained had weighted mean and numerical average molecular mass of 388 kD and 134 kD, respectively, P 32 mm Hg, arteriovenous difference 5.1 vol%, Hill's coefficient 2.14, oxygen output rate constant 55 sec, and it retained its activity during long-term storage. Its solution in a concentration of 9-10 g/dl has a viscosity of 2.34, and an oncotic pressure of 240 mm water. Bovine hemoglobin polymer is nontoxic and capable of long-term circulation in the blood. Its safety and clinical effectiveness should be studied in an extended biological experiment.
血红蛋白在红细胞内聚合，随后分离出血红蛋白聚合物。所获得的聚合物的重均分子量和数均分子量分别为388 kD和134 kD，P为32 mmHg，动静脉差值为5.1体积%，希尔系数为2.14，氧输出速率常数为55秒，并且在长期储存期间保持其活性。其浓度为9 - 10 g/dl的溶液粘度为2.34，渗透压为240 mm水柱。牛血红蛋白聚合物无毒，能够在血液中长期循环。其安全性和临床有效性应在扩展的生物学实验中进行研究。
