Vital C, Merlio J P, Rivel J, Vital A, Gautris P, Beylot M, de Mascarel A, Bloch B
Department of Neuropathology, Bordeaux II University, France.
Acta Neuropathol. 1992;84(3):331-4. doi: 10.1007/BF00227828.
Three cases of primary cerebral lymphoma in acquired immunodeficiency syndrome were studied. Tumoral fragments taken at autopsy were frozen and studied by the Southern blot technique (SBT). Other tumoral fragments were fixed in formalin, embedded in paraffin and used for in situ hybridization (ISH) with biotinylated probes for DNA of Epstein-Barr virus (EBV). ISH was positive in each case with a spotty nuclear labelling of certain tumoral cells. SBT evidenced a clonal rearrangement of the immunoglobulin heavy chain gene in each case. In addition, EBV DNA was detected in each frozen fragment with only one restriction pattern, indicating that the EBV- infected cell population was a clonal expansion of a progenitor cell.
对三例获得性免疫缺陷综合征相关的原发性脑淋巴瘤病例进行了研究。尸检时获取的肿瘤组织碎片被冷冻，并采用Southern印迹技术（SBT）进行研究。其他肿瘤组织碎片用福尔马林固定，石蜡包埋，用于与针对爱泼斯坦-巴尔病毒（EBV）DNA的生物素化探针进行原位杂交（ISH）。ISH在每例中均呈阳性，某些肿瘤细胞有斑点状核标记。SBT证明每例中免疫球蛋白重链基因均发生克隆性重排。此外，在每个冷冻碎片中均检测到EBV DNA，且只有一种限制性酶切图谱，表明受EBV感染的细胞群体是祖细胞的克隆性扩增。
