Nakamoto M, Matsubara S, Miyauchi T, Obama H, Ozawa M, Muramatsu T
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
J Biochem. 1992 Sep;112(3):346-9. doi: 10.1093/oxfordjournals.jbchem.a123903.
MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute a new family of heparin-binding growth differentiation factors. The modes of expression of MK and HB-GAM during mouse development were quantitatively examined by mRNA hybridization. The following three distinct patterns of expression were observed in the brain/head region. On the 11th-13th days of gestation, MK was intensely, but HB-GAM relatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expression became weaker; and in the neonatal period, HB-GAM was intensely expressed and MK expression increased slightly. The level of HB-GAM expression was lower than that of MK in the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected in the kidney of newborn and young mice, where MK was more highly expressed. The identity of the weakly expressed MK and HB-GAM signals was confirmed by means of the polymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos (HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAM are frequently co-expressed in the same cells and anatomic regions of the fetus or new born mouse, while their modes of expression differ.
中期因子（MK）和肝素结合生长相关分子（HB-GAM）构成了一个新的肝素结合生长分化因子家族。通过mRNA杂交定量检测了MK和HB-GAM在小鼠发育过程中的表达模式。在脑/头部区域观察到以下三种不同的表达模式。在妊娠第11至13天，MK表达强烈，但HB-GAM表达相对较弱；在第15至19天，MK和HB-GAM的表达均变弱；在新生儿期，HB-GAM表达强烈，MK表达略有增加。在妊娠第11至13天，全胚胎中HB-GAM的表达水平低于MK。在新生小鼠和幼鼠的肾脏中检测到HB-GAM mRNA，其中MK表达更高。通过聚合酶链反应在新生脑（MK）、13天胚胎头部（HB-GAM）和7日龄小鼠肾脏（HB-GAM）中证实了弱表达的MK和HB-GAM信号的一致性。总之，MK和HB-GAM在胎儿或新生小鼠的相同细胞和解剖区域中经常共同表达，但其表达模式不同。
