Tsutsui J, Uehara K, Kadomatsu K, Matsubara S, Muramatsu T
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
Biochem Biophys Res Commun. 1991 Apr 30;176(2):792-7. doi: 10.1016/s0006-291x(05)80255-4.
A retinoic acid responsive gene, MK, specifies for a heparin binding factor termed midkine (MK), which is the initial member of a new protein family involved in regulation of growth and differentiation. A cDNA clone of human MK was isolated from a fetal kidney cDNA library. Human MK mRNA was expressed in PA1 teratocarcinoma cells as well as in the kidney. Sequence analysis of the cDNA clone and of a part of the genomic clone yielded the predicted protein sequence of human MK. Human and mouse MK sequences are highly conserved: 87% of amino acids are identical and all amino acid changes are conservative except for an insertion. Comparison of MK and HB-GAM/pleiotrophin (another member of the family) from various species revealed sequences conserved in the family and those specific for each protein.
一种视黄酸反应基因MK，编码一种称为中期因子（MK）的肝素结合因子，它是参与生长和分化调节的新蛋白质家族的首个成员。人MK的cDNA克隆是从胎儿肾脏cDNA文库中分离得到的。人MK mRNA在PA1畸胎瘤细胞以及肾脏中均有表达。对该cDNA克隆和部分基因组克隆进行序列分析，得出了人MK的预测蛋白质序列。人和小鼠的MK序列高度保守：87%的氨基酸相同，除了一个插入片段外，所有氨基酸变化都是保守的。对来自不同物种的MK和HB-GAM/多效生长因子（该家族的另一个成员）进行比较，揭示了该家族中保守的序列以及每种蛋白质特有的序列。
