Iurina N A, Pavlova I G
Morfologiia. 1992 Jun;102(6):48-59.
Electron microscopic methods were used to study the antero-parietal area of the cortex of newborn rats from intact mothers (I group) and rats born after intramuscular injection of desoxycorticosterone acetate (DOCA) to pregnant rats during the III trimester (II group) in dose 10 mkg/100 g of the body mass or during the II-III trimester of gestation (III group) in dose of 2 mkg/100 g. By the moment of delivery the hormone dose in the both experimental groups was gradually decreased up to 0.8 mkg/100 g of the body mass. Prenatal treatment by DOCA resulted in the following changes in the structure of hematoencephalic barrier: ultrastructural changes of endotheliocytes and pericytes showing accelerated synthetic and metabolic processes; lengthening of interendotheliocytic dense junctions, increase of the amount of astroblast peduncles; accelerated development of the basement membrane (lengthening and thickening) and differentiation of astroblasts. Signs of accelerated differentiation of glioblasts and vascular wall were much more pronounced in the neocortex of the III group of animals. However, part of the vessels were surrounded by edema. The perivascular edema was accompanied by loosening of the basement membrane, flattening of endotheliocytes, pericytes and increase of their electron density.
采用电子显微镜方法研究了来自正常母鼠的新生大鼠（I组）以及在妊娠晚期（II组）给怀孕大鼠肌肉注射醋酸脱氧皮质酮（DOCA），剂量为10微克/100克体重，或在妊娠中期至晚期（III组），剂量为2微克/100克的大鼠的大脑皮质前顶叶区域。到分娩时，两个实验组中的激素剂量逐渐降低至0.8微克/100克体重。产前用DOCA治疗导致血脑屏障结构发生以下变化：内皮细胞和周细胞的超微结构变化，显示合成和代谢过程加速；内皮细胞间紧密连接延长，成纤维细胞蒂数量增加；基底膜加速发育（延长和增厚）以及成纤维细胞分化。在III组动物的新皮质中，成胶质细胞和血管壁加速分化的迹象更为明显。然而，部分血管被水肿包围。血管周围水肿伴有基底膜疏松、内皮细胞和平周细胞扁平以及它们电子密度增加。
