Volpes A, Sammartano F, Coffaro F, Guida S, Scaglione P, Allegra A
Centro Andros Cecos Italia, Palermo.
Acta Eur Fertil. 1992 Jul-Aug;23(4):191-4.
The aim of this study was to determine the possibility of the introduction of another parameter--the hypoosmotic swelling test--in the evaluation of the freezing potentially of anonymous donor's semen sample to be utilized in an AID program. 154 donor semen samples were frozen using classic seminal parameters: HOS tests were performed but not utilized as criteria for freezeability. All specimens were thawed and another HOS test was performed. All specimens were divided into two groups on the basis of the pre freezing HOS test value--HOS tests positive (> or = 55%) and HOS test negative (< 55%)--to verify if it's possible to correlate this value to the recovery of a good motility after thawing. A further division was performed: HOS tests highly positive (> or = 70%), HOS positive (60-55%) and HOS tests negative (< 55%) but the authors did not find any statistical difference. As concerns the clinical evaluation, were considered the last 15 pregnancies achieved with 11 samples out of 22 utilized. There was not any statistical difference. The data could seem to confirm the hypothesis that it is not possible to utilize the HOS test as a predictive value of "freezeability" of human semen sample.
本研究的目的是确定引入另一个参数——低渗肿胀试验——用于评估匿名供者精液样本在供精人工授精（AID）项目中冷冻潜力的可能性。使用经典精液参数对154份供者精液样本进行冷冻：进行了低渗肿胀试验，但未将其用作冷冻能力的标准。所有样本解冻后再次进行低渗肿胀试验。根据冷冻前低渗肿胀试验值将所有样本分为两组——低渗肿胀试验阳性（≥55%）和低渗肿胀试验阴性（<55%）——以验证该值是否与解冻后良好活力的恢复相关。进一步分组为：低渗肿胀试验高度阳性（≥70%）、低渗肿胀试验阳性（60 - 55%）和低渗肿胀试验阴性（<55%），但作者未发现任何统计学差异。关于临床评估，考虑了使用的22份样本中的11份样本所实现的最后15次妊娠情况。未发现任何统计学差异。这些数据似乎证实了这样一个假设，即不可能将低渗肿胀试验用作人类精液样本“冷冻能力”的预测指标。
