Gazzinelli G, Viana I R, Bahia-Oliveira L M, Silveira A M, Queiroz C C, Carvalho O dos S, Massara C L, Fraga L A, Colley D G, Correa-Oliveira R
Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 1992;87 Suppl 4:139-42. doi: 10.1590/s0074-02761992000800020.
Crude extracts of eggs (SEA) adult worms (SWAP) or cercariae (Cerc) have been used to stimulate Peripheral Blood Mononuclear cells (PBMC) and have provided rather distinct profiles of responses in different types of patients. In general it is clear that patients with early infections respond strongly to SEA while response to SWAP are develop more slowly. As infection progresses into the more chronic phases, a general pattern is seen which leads to lower anti-SEA proliferative responses in the face of higher responses to SWAP and variable anti-cerc responsiveness. Cured not re-exposed patients express very high levels of anti-SEA proliferation. It has recently been seen that those individuals who live in endemic areas and have continued water contact, but are repeatedly stool-negative (who are presumed to have self-cured or be putatively resistant; endemic normals) are strongly responsive to antigenic extracts, particularly to SEA. Furthermore, our results show that endemic normal individuals have significantly higher IFN gamma production upon PBMC stimulation with schistosome antigens than infected individuals. With the emergence of more studies it is becoming apparent that both the intensity and the prevalence of a given area may influence or shape the general responsiveness of the population under study.
虫卵粗提物（SEA）、成虫粗提物（SWAP）或尾蚴（Cerc）已被用于刺激外周血单个核细胞（PBMC），并且在不同类型的患者中呈现出相当不同的反应特征。一般来说，很明显早期感染患者对SEA反应强烈，而对SWAP的反应发展较慢。随着感染进入更慢性阶段，会出现一种普遍模式，即面对对SWAP的较高反应和可变的抗尾蚴反应性，抗SEA增殖反应降低。治愈且未再次接触感染源的患者表现出非常高的抗SEA增殖水平。最近发现，那些生活在流行地区且持续接触水，但多次粪便检测呈阴性的个体（推测已自愈或具有假定抗性；地方性正常人）对抗抗原提取物，特别是对SEA反应强烈。此外，我们的结果表明，与感染个体相比，地方性正常个体在用血吸虫抗原刺激PBMC后产生的IFNγ显著更高。随着更多研究的出现，越来越明显的是，特定地区的感染强度和流行率可能会影响或塑造所研究人群的总体反应性。
