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Construction and expression of nonsense suppressor tRNAs which function in plant cells.

作者信息

Franklin S, Lin T Y, Folk W R

机构信息

Department of Biochemistry, University of Missouri, Columbia 65211.

出版信息

Plant J. 1992 Jul;2(4):583-8. doi: 10.1046/j.1365-313x.1992.t01-27-00999.x.

DOI:10.1046/j.1365-313x.1992.t01-27-00999.x
PMID:1344892
Abstract

An Arabidopsis thaliana L. DNA containing the tRNA(TrpUGG) gene was isolated and altered to encode the amber suppressor tRNA(TrpUAG) or the ochre suppressor tRNA(TrpUAA). These DNAs were electroporated into carrot protoplasts and tRNA expression was demonstrated by the translational suppression of amber and ochre nonsense mutations in the chloramphenicol acetyltransferase (CAT) reporter gene. DNAs encoding tRNA(TrpUAG) and tRNA(TrpUAA) nonsense suppressor tRNAs caused suppression of their cognate nonsense codons in CAT mRNAs, with the tRNA(TrpUAG) gene exhibiting the greater suppression under optimal conditions for expression of CAT. The development of these translational suppressors which function in plant cells facilitates the study of plant tRNA gene expression and will make possible the manipulation of plant protein structure and function.

摘要

相似文献

1
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2
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引用本文的文献

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