植物色氨酸转运RNA的识别元件与氨酰化作用
Identity elements and aminoacylation of plant tRNATrp.
作者信息
Ulmasov B, Topin A, Chen Z, He S H, Folk W R
机构信息
Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, Columbia, MO 65211, USA.
出版信息
Nucleic Acids Res. 1998 Nov 15;26(22):5139-41. doi: 10.1093/nar/26.22.5139.
Mutation of the Arabidopsis thaliana tRNA (Trp)(CCA) anticodon or of the A73 discriminator base greatly diminishes in vitro aminoacylation with tryptophan, indicating the importance of these nucleotides for recognition by the plant tryptophanyl-tRNA synthetase. Mutation of the tRNA (Trp)(CCA) anticodon to CUA so as to translate amber nonsense codons permits tRNA (Trp)(CCA) to be aminoacylated by A.thaliana lysyl-tRNA synthetase. Thus, translational suppression by tRNA (TRP)(CCA) observed in plant cells includes significant incorporation of lysine into protein.
拟南芥tRNA（Trp）（CCA）反密码子或A73鉴别碱基发生突变，会极大地减少色氨酸在体外的氨酰化作用，这表明这些核苷酸对于植物色氨酰-tRNA合成酶的识别至关重要。将tRNA（Trp）（CCA）反密码子突变为CUA以便翻译琥珀色无义密码子，可使tRNA（Trp）（CCA）被拟南芥赖氨酰-tRNA合成酶氨酰化。因此，在植物细胞中观察到的tRNA（TRP）（CCA）介导的翻译抑制包括赖氨酸大量掺入蛋白质中。
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