Sabanero M, Hernández A, Martínez G, Gutiérrez F
Instituto de Investigación en Biología Experimental, Facultad de Ciencias Químicas, Universidad de Guanajuato, México.
Rev Latinoam Microbiol. 1992 Oct-Dec;34(4):313-8.
The consequences of two different mutations induced with N-methyl-N-nitrosoguanidine (Strain G1) and Trifluoperazine resistance (Strain G5) in Mucor rouxii, were studied. Mutants were stable and exhibited mycelial morphology in aerobiosis. Mutants cells exhibit phenotypic characteristics of slow-growing. The mutants G1 and G5 cultures showed 16.8% and 35.3% of reduction of the growth relative to parental strain. Morphologically mycelia of mutants cell were indistinguishable from wild-type cells, except from reduction in extension and branching of hyphal that has the mutant G5. Calcofluor and FITC-Concanavalin A were used to study the distribution of new cell-wall polymers i.e. Chitin, glucans. The two G1 and G5 strains showed a uniform distribution of fluorescence over the cell surface, indicating that active deposition of new-wall material has occurred. Cellular proteins of mutants and parental strains were labeled with 14C-aminoacid mixture. The proteins pattern revealed that the majority of polypeptides synthesized by parental strain were also synthesized by mutants. It is evident the synthesis preferential of peptides with apparent M(r) > 92K, 60K, 50K, 43K, 38K and 25K. These results indicated that the primary defect of the mutation was not on cellular differentiation. It discuss phenotypic and biochemistry characteristics from mutants.
研究了用N-甲基-N-亚硝基胍（菌株G1）和三氟拉嗪抗性（菌株G5）在鲁氏毛霉中诱导产生的两种不同突变的后果。突变体是稳定的，在需氧条件下呈现菌丝形态。突变体细胞表现出生长缓慢的表型特征。与亲本菌株相比，突变体G1和G5培养物的生长分别降低了16.8%和35.3%。除了突变体G5的菌丝延伸和分支减少外，突变体细胞的菌丝在形态上与野生型细胞没有区别。用荧光增白剂和异硫氰酸荧光素-伴刀豆球蛋白A研究了新细胞壁聚合物（即几丁质、葡聚糖）的分布。G1和G5两个菌株在细胞表面均呈现均匀的荧光分布，表明新壁物质已发生了活性沉积。用14C-氨基酸混合物对突变体和亲本菌株的细胞蛋白质进行标记。蛋白质图谱显示，亲本菌株合成的大多数多肽也由突变体合成。明显优先合成了表观分子量大于92K、60K、50K、43K、38K和25K的肽。这些结果表明，突变的主要缺陷不在于细胞分化。文中讨论了突变体的表型和生化特征。
