Sabanero M, Rojas E, Torres V, Farfán N, Contreras R
Instituto de Investigación en Biología Experimental Fac. Química. Universidad de Guanajuato.
Rev Latinoam Microbiol. 1991 Oct-Dec;33(4):297-303.
Mucor rouxii cells were used to examine the possible antimycotic activities of four substances: phenolamines, phenylendiamine and quinone. These substances are original structures recently synthesized. Assays in plates showed that 10(-2) M of phenolamines and phenylendiamines give rise to halos of growth inhibition. Assays in liquid media using 10(-4) M of substances showed 100% inhibition of spore germination. Specifically, the phenylendiamine showed 49% inhibition on development of mycelium. In these cells the calcofluor distribution changes, suggesting alterations in cell wall. No inhibition of growth was found using the quinone. The activity for substances were evaluated using standard antifungal benomyl. On this basis, the substance phenylendiamine it is an antimycotic active. The mechanism of action is not presently known.
酚胺、苯二胺和醌。这些物质是最近合成的原始结构。平板试验表明，10⁻²M的酚胺和苯二胺会产生生长抑制圈。在液体培养基中使用10⁻⁴M的这些物质进行试验，结果显示孢子萌发受到100%抑制。具体而言，苯二胺对菌丝体生长的抑制率为49%。在这些细胞中，荧光增白剂的分布发生变化，表明细胞壁发生了改变。使用醌未发现生长抑制现象。使用标准抗真菌剂苯菌灵对这些物质的活性进行了评估。在此基础上，苯二胺这种物质具有抗真菌活性。目前其作用机制尚不清楚。
