Tomazic V, Vitale B
Z Immunitatsforsch Exp Klin Immunol. 1976 Mar;151(1-2):22-31.
The immunological capacity of lymphoid cells from mice rendered tolerant to high and low doses of BSA was investigated. The tolerance was induced by multiple injections of high and low doses of antigen through the period of 30 days. Lymph node and bone marrow cells from tolerant animals were transferred into lethally irradiated syngeneic recipients. After 10 days, when lymphoid organs of the recipients were repopulated with the injected cells, challenge injection of the same antigen incorporated into complete Freund's adjuvant was given. The immune response of the transferred cells in the recipients was evaluated by analysis of the specific antibodies in the sera. Lymphoid cells from donors rendered toloerant with high doses of antigen recovered their reactivity 20 days after the transfer to the level of reaction of normal cells. Lymphoid cells from donors receiving multiple injection of low doses of BSA remained tolerant after the transfer through the entire observation period. According to the cellular events in the donors during the period of tolerance induction, and the behaviour of the transferred lymphoid cells in the new recipients, it seems possible that tolerance induced with high doses of BSA corresponded to the B-cell tolerance, while low doses of antigen most likely induced tolerance of T-cell population. The possible cellular mechanisms of B and T-cell tolerance were discussed.
研究了对高剂量和低剂量牛血清白蛋白（BSA）产生耐受的小鼠淋巴细胞的免疫能力。通过在30天内多次注射高剂量和低剂量抗原诱导耐受。将来自耐受动物的淋巴结和骨髓细胞转移到经致死剂量照射的同基因受体中。10天后，当受体的淋巴器官被注入的细胞重新填充时，给予掺入完全弗氏佐剂的相同抗原的激发注射。通过分析血清中的特异性抗体来评估受体中转移细胞的免疫反应。来自用高剂量抗原诱导耐受的供体的淋巴细胞在转移后20天恢复其反应性，达到正常细胞反应水平。接受多次低剂量BSA注射的供体的淋巴细胞在转移后的整个观察期内仍保持耐受。根据耐受诱导期供体中的细胞事件以及转移的淋巴细胞在新受体中的行为，高剂量BSA诱导的耐受可能对应于B细胞耐受，而低剂量抗原最有可能诱导T细胞群体的耐受。讨论了B细胞和T细胞耐受的可能细胞机制。
