抗氢化链霉菌20β-羟基类固醇脱氢酶抗血清的特性
Properties of an antiserum against Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase.
作者信息
Lotz B, Betz J, Träger L
出版信息
Z Naturforsch C Biosci. 1976 May-Jun;31(5-6):288-91. doi: 10.1515/znc-1976-5-613.
PMID:134571
Abstract
Antiserum against crystallized 20beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20beta-hydroxysteroid dehydrogenase was calculated to be 27 300 +/- 700.
摘要
利用抗氢化链霉菌结晶20β-羟基类固醇脱氢酶的抗血清进行不同的免疫扩散和免疫沉淀试验，以定量该微生物无细胞上清液中的细菌酶。经过免疫沉淀和凝胶电泳后，计算出20β-羟基类固醇脱氢酶亚基的分子量为27300±700。
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