Brahmachary R L, Ghosal D
Z Naturforsch C Biosci. 1976 Jul-Aug;31(7-8):488. doi: 10.1515/znc-1976-7-829.
An attempt has been made to partially characterize the substance responsible for the rhythmic incorporation of 35S (inorganic sulfate) in the course of the cell cycle in early Limnaea embryos. This trichloroacetic acid (TCA) insoluble substance is partly pronase sensitive, and dissociable into two fractions after treatment with NaCl. One of these remains TCA-insoluble while the other is TCA soluble but precipitable by cetyl pyridinium chloride. Thus, unlike in some other higher organisms, the major part of the inorganic sulfate is incorporated here into a fraction which is not a single mycopolysaccharide, but is more likely to be a protein-mucopolysaccharide complex, rather like the chondromucoprotein of chick-embryo cartilage.
人们试图对负责在早期椎实螺胚胎细胞周期中35S（无机硫酸盐）有节奏地掺入的物质进行部分表征。这种不溶于三氯乙酸（TCA）的物质部分对链霉蛋白酶敏感，用氯化钠处理后可解离成两部分。其中一部分仍不溶于TCA，而另一部分可溶于TCA，但可被十六烷基氯化吡啶沉淀。因此，与其他一些高等生物不同，这里大部分无机硫酸盐掺入的部分不是单一的霉菌多糖，而更可能是一种蛋白质 - 粘多糖复合物，有点像鸡胚软骨的软骨粘蛋白。
