氮调节蛋白I（NtrC）作为肠道细菌中谷氨酰胺合成酶基因（glnA）的转录激活因子，在氮限制生长期间降低glnA表达中的作用。
Role of nitrogen regulator I (NtrC), the transcriptional activator of glnA in enteric bacteria, in reducing expression of glnA during nitrogen-limited growth.
作者信息
Shiau S P, Schneider B L, Gu W, Reitzer L J
机构信息
Program in Molecular and Cell Biology, University of Texas at Dallas, Richardson 75083-0688.
出版信息
J Bacteriol. 1992 Jan;174(1):179-85. doi: 10.1128/jb.174.1.179-185.1992.
Abstract
During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular concentration of NRI lowered the level of glutamine synthetase. The reduction in glutamine synthetase does not appear to result from the NRI-dependent sequestering of any protein that affects transcription of glnA. Our results also suggest that the negative effect of a high concentration of NRI on glnA expression is a major determinant of the level of glutamine synthetase activity in nitrogen-limited cells of a wild-type strain. We propose that the inhibition results from an impairment of the interaction between NRI-phosphate and RNA polymerase that stimulates glnA transcription. We discuss a model that can account for this reduction in glutamine synthetase.
摘要
在氮限制生长期间，编码谷氨酰胺合成酶的glnA的转录需要σ54-RNA聚合酶以及来自氮调节因子I（NRI；也称为NtrC）的磷酸化形式。在lac启动子控制NRI编码基因表达的细胞中，增加细胞内NRI的浓度会降低谷氨酰胺合成酶的水平。谷氨酰胺合成酶的减少似乎不是由于NRI依赖的对任何影响glnA转录的蛋白质的隔离。我们的结果还表明，高浓度NRI对glnA表达的负面影响是野生型菌株氮限制细胞中谷氨酰胺合成酶活性水平的主要决定因素。我们提出这种抑制是由于刺激glnA转录的NRI-磷酸与RNA聚合酶之间的相互作用受损所致。我们讨论了一个可以解释谷氨酰胺合成酶这种减少的模型。
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本文引用的文献
1
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Proc Natl Acad Sci U S A. 1983 Sep;80(18):5554-8. doi: 10.1073/pnas.80.18.5554.
2
Gene regulation at the right operator (OR) of bacteriophage lambda. II. OR1, OR2, and OR3: their roles in mediating the effects of repressor and cro.λ噬菌体右操纵基因（OR）处的基因调控。II. OR1、OR2和OR3：它们在介导阻遏蛋白和Cro蛋白作用中的角色
J Mol Biol. 1980 May 15;139(2):163-94. doi: 10.1016/0022-2836(80)90303-4.
3
Tandem promoters determine regulation of the Klebsiella pneumoniae glutamine synthetase (glnA) gene.串联启动子决定肺炎克雷伯菌谷氨酰胺合成酶（glnA）基因的调控。
Nucleic Acids Res. 1984 Oct 25;12(20):7811-30. doi: 10.1093/nar/12.20.7811.
4
Identification and regulation of the glnL operator-promoter of the complex glnALG operon of Escherichia coli.大肠杆菌复杂的谷氨酰胺合成酶基因操纵子（glnALG operon）中谷氨酰胺合成酶基因L（glnL）操纵子-启动子的鉴定与调控
J Bacteriol. 1984 Oct;160(1):379-84. doi: 10.1128/jb.160.1.379-384.1984.
5
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EMBO J. 1984 Mar;3(3):501-7. doi: 10.1002/j.1460-2075.1984.tb01837.x.
6
Evidence that nitrogen regulatory gene ntrC of Salmonella typhimurium is transcribed from the glnA promoter as well as from a separate ntr promoter.有证据表明，鼠伤寒沙门氏菌的氮调节基因ntrC是从谷氨酰胺合成酶基因（glnA）启动子以及一个独立的ntr启动子转录而来的。
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7
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J Bacteriol. 1983 Mar;153(3):1247-51. doi: 10.1128/jb.153.3.1247-1251.1983.
8
Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.一个基因（glnL）的特性，其产物参与大肠杆菌氮利用的调控。
J Bacteriol. 1982 Apr;150(1):214-20. doi: 10.1128/jb.150.1.214-220.1982.
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J Bacteriol. 1982 Apr;150(1):202-13. doi: 10.1128/jb.150.1.202-213.1982.
10
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.大肠杆菌染色体谷氨酰胺合成酶基因（glnA）-谷氨酰胺合成酶基因激活蛋白基因（glnG）区域的物理和遗传特征分析
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3743-7. doi: 10.1073/pnas.78.6.3743.
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