Wang W T, Lundgren T, Lindh F, Nilsson B, Grönberg G, Brown J P, Mentzer-Dibert H, Zopf D
BioCarb, Inc., Gaithersburg, Maryland 20879.
Arch Biochem Biophys. 1992 Feb 1;292(2):433-41. doi: 10.1016/0003-9861(92)90013-m.
A monoclonal antibody, Onc-M26, that recognizes a cancer-associated antigen expressed by most human adenocarcinomas of the breast was shown previously to recognize a carbohydrate epitope carried on a hexaglycosyl ganglioside carrying the sialyl-Lewis X (SLex) antigen (P.S. Linsley et al., 1988, Cancer Res. 48, 2138-2148). Evidence that the antibody binds even more avidly to minor gangliosides containing more complex carbohydrate chains prompted us to search for a higher affinity epitope among sialylated oligosaccharides from pooled human milk. Affinity chromatography of a partially purified fraction of monosialylated milk oligosaccharides on a column containing monoclonal antibody Onc-M26 bound to a macroporous silica matrix gave a peak with a retention volume significantly greater than that of a standard SLex-active hexasaccharide. The retained material consisted of two nonasaccharides, each containing the SLex tetrasaccharide sequence, Neu5Ac alpha 2-3Gal beta 1-4(Fuc alpha 1-3) GlcNAc, linked beta 1-6 to a 3,6-disubstituted galactosyl residue.
一种单克隆抗体Onc-M26，它能识别大多数人乳腺腺癌所表达的一种癌症相关抗原，先前已证明它能识别一种带有唾液酸化路易斯X（SLex）抗原的六糖基神经节苷脂上携带的碳水化合物表位（P.S.林斯里等人，1988年，《癌症研究》48卷，2138 - 2148页）。有证据表明该抗体与含有更复杂碳水化合物链的次要神经节苷脂结合得更为紧密，这促使我们在人乳混合唾液酸化寡糖中寻找更高亲和力的表位。将部分纯化的单唾液酸化乳寡糖级分在含有与大孔硅胶基质结合的单克隆抗体Onc-M26的柱上进行亲和层析，得到一个保留体积明显大于标准SLex活性六糖的峰。保留的物质由两种九糖组成，每种都含有SLex四糖序列，即Neu5Acα2 - 3Galβ1 - 4（Fucα1 - 3）GlcNAc，通过β1 - 6连接到一个3,6 - 二取代半乳糖基残基上。
