Kitagawa H, Nakada H, Fukui S, Funakoshi I, Kawasaki T, Yamashina I, Tate S, Inagaki F
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Biochemistry. 1991 Mar 19;30(11):2869-76. doi: 10.1021/bi00225a020.
We have isolated four novel oligosaccharides with the sialyl-Lea structure from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. The results of structural analyses, i.e., 500-MHz 1H NMR spectroscopy, fast atom bombardment mass spectrometry, and binding to specific anticarbohydrate antibodies, are consistent with the following structures. (formula; see text)
我们使用单克隆抗体MSW 113从人乳中分离出了四种具有唾液酸化-Lea结构的新型寡糖。这些寡糖通过固定化单克隆抗体柱上的亲和色谱法和高效液相色谱法进行了纯化。结构分析的结果，即500兆赫兹的1H核磁共振光谱、快原子轰击质谱以及与特定抗碳水化合物抗体的结合，与以下结构一致。（分子式；见正文）
