Kitagawa H, Nakada H, Fukui S, Funakoshi I, Kawasaki T, Yamashina I, Tate S, Inagaki F
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
J Biochem. 1993 Oct;114(4):504-8. doi: 10.1093/oxfordjournals.jbchem.a124207.
Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized by a combination of 600-MHz 1H NMR spectroscopy and fast atom bombardment mass spectrometry. The structural studies indicated the structures of these oligosaccharides to be: [formula: see text]
使用单克隆抗体MSW 113从人乳中分离出两种具有唾液酸化-Le(a)结构的新型寡糖。这些寡糖通过固定化单克隆抗体柱上的亲和色谱法和高效液相色谱法进行纯化，并通过600兆赫兹1H核磁共振光谱和快原子轰击质谱联用进行结构表征。结构研究表明这些寡糖的结构为：[化学式：见原文]
