用于扩增片段长度多态性分析的可重复等位基因标记的扩增
Amplification of reproducible allele markers for amplified fragment length polymorphism analysis.
作者信息
Sajantila A, Puomilahti S, Johnsson V, Ehnholm C
机构信息
Laboratory for Forensic Serology, National Public Health Institute, Helsinki, Finland.
出版信息
Biotechniques. 1992 Jan;12(1):16, 18, 20-2.
A procedure for amplification by PCR of reproducible allele markers for amplified fragment length polymorphism (Amp-FLP) analysis is presented. We have prepared markers for the allelic products of the VNTR loci D1S80 (MCT118) and D17S30 (YNZ22) and for the hypervariable VNTR locus close to the 3' end of the apolipoprotein B gene (apoB) by re-amplifying a mixture of PCR products from individuals with known alleles. These allele markers allow precise and discrete determination of the VNTR alleles at these loci using the Amp-FLP technique that should prove suitable in forensic analyses, paternity testing and population studies.
本文介绍了一种通过聚合酶链反应（PCR）扩增用于扩增片段长度多态性（Amp-FLP）分析的可重复等位基因标记的方法。我们通过对已知等位基因个体的PCR产物混合物进行再扩增，制备了VNTR位点D1S80（MCT118）和D17S30（YNZ22）的等位基因产物标记，以及靠近载脂蛋白B基因（apoB）3'端的高变VNTR位点的标记。这些等位基因标记允许使用Amp-FLP技术精确且离散地确定这些位点的VNTR等位基因，该技术在法医分析、亲子鉴定和群体研究中应证明是适用的。
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