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聚合酶链反应在法医DNA分析中鉴定D1S80(pMCT 118)基因座的应用

[Applications of polymerase chain reaction in identifying the D1S80 (pMCT 118) locus in forensic DNA analysis].

作者信息

Huber P, Holtz J

机构信息

Institut für Rechtsmedizin, Friedrich-Wilhelms-Universität zu Bonn.

出版信息

Arch Kriminol. 1994 Jul-Aug;194(1-2):47-54.

PMID:7979868
Abstract

The value of the amplified fragment length polymorphism at the D1S80 locus was examples by means of examples of forensic casework (paternity and identification cases). Parameters, like PCR conditions, limit of detection and analysis of mixed samples are discussed. In a population study the allele and genotype frequencies of 154 individuals from the area of Bonn were determined. The distribution of genotypes was in agreement with expected values according to the Hardy-Weinberg equilibrium. There was no deviation from other published Middle European studies.

摘要

通过法医案例工作(亲子鉴定和身份鉴定案例)实例说明了D1S80位点扩增片段长度多态性的价值。讨论了诸如PCR条件、检测限和混合样本分析等参数。在一项群体研究中,确定了来自波恩地区154名个体的等位基因和基因型频率。基因型分布符合哈迪-温伯格平衡的预期值。与其他已发表的中欧研究结果没有偏差。

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