Yokoyama S, Yamakawa K, Tsuchiya E, Murata M, Sakiyama S, Nakamura Y
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Cancer Res. 1992 Feb 15;52(4):873-7.
We examined loss of heterozygosity in 49 adenocarcinomas and 18 squamous cell carcinomas of the lung with 19 RFLP markers on the short arm of chromosome 3. Although no interstitial deletions were observed in any squamous cell carcinomas, interstitial or partial deletions were detected in 23 adenocarcinomas. Identification of two common regions of deletion in adenocarcinomas, at 3p21.3 and 3p14.1-21.1, suggested the presence of at least two tumor suppressor genes on 3p within the same regions commonly deleted in renal cell carcinomas. Correlation between the frequency of loss of heterozygosity on 3p and histopathological grade of adenocarcinoma also was observed. These results imply an etiological difference between two major types of non-small cell lung cancers, adenocarcinoma and squamous cell carcinoma.
我们用位于3号染色体短臂上的19个限制性片段长度多态性（RFLP）标记，检测了49例肺腺癌和18例肺鳞状细胞癌中的杂合性缺失。尽管在任何鳞状细胞癌中均未观察到间质缺失，但在23例腺癌中检测到了间质或部分缺失。在腺癌中3p21.3和3p14.1 - 21.1处发现两个常见的缺失区域，这表明在3p上与肾细胞癌常见缺失区域相同的区域内至少存在两个肿瘤抑制基因。还观察到了3p杂合性缺失频率与腺癌组织病理学分级之间的相关性。这些结果提示非小细胞肺癌的两种主要类型，即腺癌和鳞状细胞癌之间存在病因学差异。
