Signorini M, Mariotti E, Bergamini C M
Istituto di Chimica Biologica, Università di Ferrara, Italy.
Biol Chem Hoppe Seyler. 1992 Jan;373(1):21-5. doi: 10.1515/bchm3.1992.373.1.21.
Acrylamide rapidly and irreversibly inactivates thrombin activated Factor XIIIa, without affecting neither the intact zymogen nor its proteolytic activation. The inactivation is strictly dependent on the presence of calcium ions and is accompanied by a decrease in the number of free and total thiol residues, suggesting that cysteine residue(s), whose reactivity is modulated by calcium, is (are) probably responsible for the acrylamide-directed inactivation.
丙烯酰胺能迅速且不可逆地使凝血酶激活的因子XIIIa失活，而不影响完整的酶原及其蛋白水解激活过程。这种失活严格依赖于钙离子的存在，同时伴随着游离巯基和总巯基残基数量的减少，这表明其反应性受钙离子调节的半胱氨酸残基可能是丙烯酰胺定向失活的原因。
