Bergamini C M, Signorini M
Istitute of Biological Chemistry, University of Ferrara, Italy.
Biochem Int. 1988 Nov;17(5):855-62.
Purified transglutaminase from human erythrocytes was shown to undergo time dependent inactivation by acrylamide added at millimolar concentration: this effect was wholly dependent on calcium ions and some protection was produced by glutamine substrates and GTP. The enzyme activity was recovered by addition of thiol based reducing agents, while ascorbic acid and sodium borohydrate were ineffective. These data are suggestive of an active site directed action of acrylamide.
已表明，来自人红细胞的纯化转谷氨酰胺酶会被以毫摩尔浓度添加的丙烯酰胺随时间而失活：这种效应完全依赖于钙离子，谷氨酰胺底物和GTP可产生一定程度的保护作用。通过添加基于硫醇的还原剂可恢复酶活性，而抗坏血酸和硼氢化钠则无效。这些数据提示丙烯酰胺存在活性位点导向作用。
