使用带有小鼠DNA插入片段的酵母人工染色体文库进行基因组分析。
Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.
作者信息
Rossi J M, Burke D T, Leung J C, Koos D S, Chen H, Tilghman S M
机构信息
Howard Hughes Medical Institute, Princeton University, NJ 08544.
出版信息
Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2456-60. doi: 10.1073/pnas.89.6.2456.
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
一个含有小鼠基因组DNA插入片段的酵母人工染色体文库已经构建完成。该文库覆盖了小鼠基因组2.5倍的范围，平均插入片段大小为250千碱基对。筛选策略是对从单独保存的克隆中制备的混合DNA进行聚合酶链反应。通过构建围绕Hba-ps4的600千碱基对长的DNA重叠群，证明了该文库在染色体步移中的实用性，Hba-ps4是一个与17号染色体上的融合（Fu）位点紧密连锁的DNA标记。
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