Blankenhorn E P, Smith P D, Williams C B, Gutman G A
Department of Microbiology and Immunology, Hahnemann University, Philadelphia, PA 19102-1192.
Immunogenetics. 1992;35(5):324-31. doi: 10.1007/BF00189895.
Inbred rat strains provide a rich source of genetic diversity in immunologically relevant genes. We have characterized the alleles of one of these genes, encoding the rat T-cell receptor C beta 1 chain, by Southern blots and nucleic acid sequencing. The Cb1 gene segments from DA and LEW rats display complex allotypic variation: both coding and noncoding regions contain multiple nucleotide substitutions. In addition, there is a polymorphic insertion of a rat repetitive LINE element 3' to the coding region. The Cb1 alleles are one part of larger Tcrb haplotypes, containing V beta, D beta, and J beta elements; complete Cb1 genomic nucleotide sequences, and a partial list of the strain distribution of the two alleles, are described in this report.
近交系大鼠品系在免疫相关基因方面提供了丰富的遗传多样性来源。我们通过Southern印迹法和核酸测序对其中一个编码大鼠T细胞受体Cβ1链的基因的等位基因进行了表征。来自DA和LEW大鼠的Cb1基因片段表现出复杂的同种异型变异：编码区和非编码区都含有多个核苷酸替换。此外，在编码区3'端有一个大鼠重复LINE元件的多态性插入。Cb1等位基因是较大的Tcrb单倍型的一部分，包含Vβ、Dβ和Jβ元件；本报告描述了完整的Cb1基因组核苷酸序列以及这两个等位基因的品系分布部分列表。
