Malissen M, Minard K, Mjolsness S, Kronenberg M, Goverman J, Hunkapiller T, Prystowsky M B, Yoshikai Y, Fitch F, Mak T W
Cell. 1984 Jul;37(3):1101-10. doi: 10.1016/0092-8674(84)90444-6.
The germ-line joining (J) gene segments and constant (C) genes encoding the beta chain of the mouse T cell antigen receptor have been isolated on a single cosmid clone. There are two constant genes, C beta 1 and C beta 2, each associated with a cluster of J beta gene segments. The nucleotide sequences of the C beta 2 gene and of the J beta 2 cluster gene segments have been determined. The coding sequence of the C beta 2 gene is very similar to the sequence of a cDNA clone encoded by the C beta 1 gene. The C beta 2 gene has four exons; exon-intron structure does not obviously correspond to the functional domains of the protein. The J beta 2 gene segment cluster contains six functional J gene segments. We have isolated specific probes for the C beta 1, C beta 2, J beta 1, and J beta 2 regions to examine DNA rearrangements in T lymphocytes. DNA rearrangements can occur in both J beta gene segment clusters, and both C beta genes appear functional.
编码小鼠T细胞抗原受体β链的种系连接（J）基因片段和恒定（C）基因已在一个单一黏粒克隆上分离出来。有两个恒定基因，Cβ1和Cβ2，每个都与一个Jβ基因片段簇相关联。已确定Cβ2基因和Jβ2簇基因片段的核苷酸序列。Cβ2基因的编码序列与由Cβ1基因编码的一个cDNA克隆的序列非常相似。Cβ2基因有四个外显子；外显子-内含子结构与蛋白质的功能结构域没有明显对应关系。Jβ2基因片段簇包含六个功能性J基因片段。我们已分离出针对Cβ1、Cβ2、Jβ1和Jβ2区域的特异性探针，以检测T淋巴细胞中的DNA重排。DNA重排在两个Jβ基因片段簇中都可能发生，并且两个Cβ基因似乎都有功能。
