Jeffery J R, Guttmann R D, Charpentier B
Clin Exp Immunol. 1976 Sep;25(3):437-41.
Fourteen cadaver kidney donors were pretreated with a rapid infusion of 5 g of cyclophosphamide and 5 g of methylprednisolone in an attempt to reduce graft immunogenicity by altering immunogenic "passenger" leucocytes. The effects of this drug infusion on peripheral lymphocytes were monitored by measuring the ability of donor lymphocytes to respond and to stimulate recipient cells in mixed lymphocyte culture and by measuring their ability to respond to phytohaemagglutinin stimulation before and after infusion of the drugs. Pretreatment severely decreased the ability of donor cells to function in these tests, but the maximum effect was not seen until several hours after completion of the infusion. The results of this study suggest that donor nephrectomy should be delayed for at least 4 hr after pretreatment to obtain maximum lymphocyte effect using this regimen. This type of in vitro testing may provide means of monitoring donor pretreatment and comparing different pretreatment protocols in human cadaveric allotransplantation.
十四名尸体肾供者接受了快速输注5克环磷酰胺和5克甲泼尼龙的预处理，试图通过改变具有免疫原性的“过客”白细胞来降低移植物的免疫原性。通过测量供体淋巴细胞在混合淋巴细胞培养中对受体细胞作出反应和刺激的能力，以及测量药物输注前后供体淋巴细胞对植物血凝素刺激作出反应的能力，来监测这种药物输注对外周淋巴细胞的影响。预处理严重降低了供体细胞在这些测试中的功能，但直到输注完成数小时后才出现最大效果。本研究结果表明，采用该方案进行预处理后，供肾切除应至少延迟4小时，以获得最大的淋巴细胞效应。这种体外检测方法可能为监测供体预处理情况以及比较人类尸体同种异体移植中不同的预处理方案提供手段。
