硫代硫酸盐嗜绿菌中一种三磷酸腺苷酶的定位及可能作用
Localization and possible role of an adenosine triphosphatase in Chlorobium thiosulfatophilum.
作者信息
Burns D D, Midgley M
出版信息
Eur J Biochem. 1976 Aug 16;67(2):323-33. doi: 10.1111/j.1432-1033.1976.tb10695.x.
Abstract
- Evidence is presented that the ATPase activity detected in cell extracts of Chlorobium thiosulfatophilum is bound to the cytoplasmic membrane rather than to the chlorobium vesicles. 2. The activity of this ATPase is inhibited in vitro by various carbodiimides, phloridzin and sodium azide. 3. The apparent Km for ATP is approximately 0.2 mM and the enzyme shows product inhibition by ADP. 4. Photophosphorylation, characterized in vivo, is inhibited by many of the compounds that inhibit the ATPase.
摘要
- 有证据表明，在嗜硫绿菌细胞提取物中检测到的ATP酶活性与细胞质膜结合，而非与绿菌囊泡结合。2. 这种ATP酶的活性在体外受到各种碳二亚胺、根皮苷和叠氮化钠的抑制。3. ATP的表观米氏常数约为0.2 mM，该酶表现出受ADP的产物抑制作用。4. 体内特征化的光合磷酸化受到许多抑制ATP酶的化合物的抑制。
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