Doddema H J, van der Drift C, Vogels G D, Veenhuis M
J Bacteriol. 1979 Dec;140(3):1081-9. doi: 10.1128/jb.140.3.1081-1089.1979.
Hydrogenase and the adenosine 5'-triphosphate (ATP) synthetase complex, two enzymes essential in ATP generation in Methanobacterium thermoautotrophicum, were localized in internal membrane systems as shown by cytochemical techniques. Membrane vesicles from this organism possessed hydrogenase and adenosine triphosphatase (ATPase) activity and synthesized ATP driven by hydrogen oxidation or a potassium gradient. ATP synthesis depended on anaerobic conditions and could be inhibited in membrane vesicles by uncouplers, nigericin, or the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. The presence of an adenosine 5'-diphosphate-ATP translocase was postulated. With fluorescent dyes, a membrane potential and pH gradient were demonstrated.
氢化酶和腺苷5'-三磷酸（ATP）合成酶复合体是嗜热自养甲烷杆菌中ATP生成所必需的两种酶，细胞化学技术显示它们定位于内膜系统中。来自该生物体的膜泡具有氢化酶和腺苷三磷酸酶（ATP酶）活性，并能在氢气氧化或钾离子梯度驱动下合成ATP。ATP的合成依赖于厌氧条件，并且在膜泡中可被解偶联剂、尼日利亚菌素或ATP酶抑制剂N，N'-二环己基碳二亚胺抑制。推测存在腺苷5'-二磷酸-ATP转位酶。利用荧光染料证明了膜电位和pH梯度的存在。
