Sugitani M, Inchauspé G, Shindo M, Prince A M
First Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Lancet. 1992 Apr 25;339(8800):1018-9. doi: 10.1016/0140-6736(92)90538-e.
Blood donors at high risk of hepatitis C virus (HCV) infection were tested for viraemia by the polymerase chain reaction (PCR). PCR results were accepted as positive only if reactive in 3 of 4 tests and if confirmed in an independent laboratory. The sera were also tested by 6 different assays to determine the ability of current serological assays to detect viraemic blood donors. Of 19 PCR-positive sera, only 13 (68%) were detected by the most sensitive of the serological assays. If these results are confirmed, automated PCR assays may be required for blood-donor screening to prevent transmission of HCV.
采用聚合酶链反应（PCR）对丙型肝炎病毒（HCV）感染高危献血者进行病毒血症检测。仅当4次检测中有3次呈反应性且经独立实验室确认时，PCR结果才被判定为阳性。还通过6种不同检测方法对血清进行检测，以确定当前血清学检测方法检测病毒血症献血者的能力。在19份PCR阳性血清中，最敏感的血清学检测方法仅检测出13份（68%）。如果这些结果得到证实，可能需要采用自动化PCR检测方法进行献血者筛查，以防止HCV传播。
