Buffet C, Charnaux N, Laurent-Puig P, Chopineau S, Quichon J P, Briantais M J, Dussaix E
Service des Maladies due Foie et de L'Appareil Digestif, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.
J Med Virol. 1994 Jul;43(3):259-61. doi: 10.1002/jmv.1890430312.
Fifty-seven sera with indeterminate results by the second generation RIBA (RIBA 2) for confirmation of hepatitis C virus (HCV) enzyme linked immunoassay (ELISA) reactivity were tested by the new third generation RIBA (RIBA 3). Thirty three (57.9%) displayed reactivity for at least one other band and were therefore classified as positive; two became negative and 22 (38%) remained indeterminate. The incidence of HCV viremia, as determined by the RNA polymerase chain reaction (PCR), was 75% for the latter sera. The data show that it is important to subject RIBA 3 indeterminate samples to PCR.
57份经第二代重组免疫印迹法（RIBA 2）检测结果不确定、用于确认丙型肝炎病毒（HCV）酶联免疫吸附测定（ELISA）反应性的血清，采用新型第三代重组免疫印迹法（RIBA 3）进行检测。其中33份（57.9%）至少出现一条其他条带的反应性，因此被分类为阳性；2份变为阴性，22份（38%）仍为不确定结果。通过RNA聚合酶链反应（PCR）测定，后一组血清中HCV病毒血症的发生率为75%。数据表明，对RIBA 3不确定的样本进行PCR检测很重要。
