无症状献血者中的丙型肝炎病毒抗体与病毒复制

Hepatitis C virus antibodies and virus replication in asymptomatic blood donors.

作者信息

Yuki N, Hayashi N, Hagiwara H, Naito M, Ohkawa K, Kasahara A, Fusamoto H, Ohtani S, Okubo Y, Kamada T

机构信息

First Department of Medicine, Osaka University Medical School, Suita, Japan.

出版信息

Vox Sang. 1994;67(3):280-5. doi: 10.1111/j.1423-0410.1994.tb01252.x.

DOI:10.1111/j.1423-0410.1994.tb01252.x

PMID:7532341

Abstract

We assessed hepatitis C virus (HCV) infection in 99 asymptomatic blood donors positive using a first-generation HCV antibody assay. When tested with second-generation assays, 86 (87%) donors were reactive (group 1), 2 (2%) were indeterminate (group 2), and 11 (11%) were non-reactive (group 3). Viraemia was revealed by polymerase chain reaction in all group 1 cases. The 2 group 2 cases and 6 (55%) group 3 cases were also viraemic. Viraemia was confirmed by a branched DNA assay in the 2 group 2 cases and 4 (36%) group 3 cases. Serum HCV RNA levels were further studied using a competitive reverse transcription-polymerase chain reaction assay. All cases in groups 2 and 3 were low viraemic (range 10(4)-10(5.5) copies/ml) compared with the 9 group 1 cases examined (range 10(7)-10(9) copies/ml). No correlation was evident between viraemic levels and antibody cut-off index in the first-generation assay. These findings indicate the possibility that low levels of viraemia can occur in individuals non-reactive in second-generation HCV antibody assays.

摘要

我们使用第一代丙型肝炎病毒（HCV）抗体检测法对99名无症状的献血者进行了HCV感染评估。当用第二代检测法检测时，86名（87%）献血者呈反应性（第1组），2名（2%）结果不确定（第2组），11名（11%）无反应（第3组）。通过聚合酶链反应在所有第1组病例中发现了病毒血症。第2组的2例和第3组的6例（55%）也存在病毒血症。通过分支DNA检测法在第2组的2例和第3组的4例（36%）中确认了病毒血症。使用竞争性逆转录-聚合酶链反应检测法进一步研究血清HCV RNA水平。与检测的第1组的9例（范围为10(7)-10(9)拷贝/毫升）相比，第2组和第3组的所有病例病毒血症水平较低（范围为10(4)-10(5.5)拷贝/毫升）。在第一代检测中，病毒血症水平与抗体临界指数之间没有明显的相关性。这些发现表明，在第二代HCV抗体检测中无反应的个体可能出现低水平病毒血症。