Saha K, Wong P K
University of Texas, M. D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Virology. 1992 Jun;188(2):921-5. doi: 10.1016/0042-6822(92)90554-3.
We examined the role of T cells and their separated subsets in providing immunity against ts1 (a mutant of the Moloney murine leukemia virus) induced paralysis and immunodeficiency. Adoptive transfer of syngeneic total T cells from immunized mice protected newborn mice, at least partially, from ts1-induced disease syndrome. In infected mice who received total immune T cells, virus replication was reduced and the mice survived longer. When only separated immune CD8+ T cells were transferred to infected mice, similar protection, albeit to a lesser extent, was observed. Transfer of separated immune CD4+ T cells alone gave no protection. However, when recombined CD4+ and CD8+ cells were transferred together, an immune response similar to that when total T cells were transferred was observed. Cytotoxic assays from ts1-immunized mice revealed the presence of virus-specific CD8+ cytotoxic T lymphocytes that could lyse virus-expressing cells at a high effector/target ratio. We conclude that CD8+ T cells alone can provide immunity against ts1-induced paralysis and immunodeficiency and that the simultaneous presence of CD4+ T cells can also significantly enhance the immune response.
我们研究了T细胞及其分离的亚群在抵抗ts1（莫洛尼鼠白血病病毒的一种突变体）诱导的麻痹和免疫缺陷方面所起的作用。将免疫小鼠的同基因总T细胞进行过继转移，可至少部分地保护新生小鼠免受ts1诱导的疾病综合征影响。在接受总免疫T细胞的感染小鼠中，病毒复制减少，小鼠存活时间延长。当仅将分离的免疫CD8⁺ T细胞转移到感染小鼠时，观察到了类似的保护作用，尽管程度较轻。单独转移分离的免疫CD4⁺ T细胞则没有保护作用。然而，当将重组的CD4⁺ 和CD8⁺ 细胞一起转移时，观察到了与转移总T细胞时相似的免疫反应。对ts1免疫小鼠进行的细胞毒性测定显示，存在病毒特异性CD8⁺ 细胞毒性T淋巴细胞，它们能够以高效应细胞/靶细胞比例裂解表达病毒的细胞。我们得出结论，单独的CD8⁺ T细胞即可提供针对ts1诱导的麻痹和免疫缺陷的免疫力，并且CD4⁺ T细胞的同时存在也可显著增强免疫反应。
