Shirahama T, Ikoma M, Muramatsu H, Muramatsu T, Ohi Y
Department of Urology and Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
J Urol. 1992 Jun;147(6):1659-64. doi: 10.1016/s0022-5347(17)37673-5.
Expression of binding sites for fucose binding proteins (FBP) of Lotus tetragonolobus were immunohistochemically analyzed in surgically extirpated specimens from patients with transitional cell carcinoma of the bladder. The degree of expression of FBP binding sites in the primary cancerous region correlated with the occurrence of lymph node metastasis. Thus, lymph node metastases occurred in 15 of 34 cases with high expression of FBP binding sites, but did not in 17 cases with low or no FBP binding site expression. All metastatic lymph nodes strongly reacted with FBP. In addition, primary lesions at the pT3b or pT4 stage more frequently reacted with FBP as compared with those at the pTa, pT1 or pT2 stage. Although FBP is known to react both with Gal beta 1----4(Fuc alpha 1----3)GlcNAc and Fuc alpha 1----2Gal sequences, comparative staining of other carbohydrate markers revealed that the latter structure is not related with metastatic potential and stages.
采用免疫组织化学方法分析了四角豆岩藻糖结合蛋白（FBP）结合位点在膀胱移行细胞癌患者手术切除标本中的表达情况。原发性癌灶区域FBP结合位点的表达程度与淋巴结转移的发生相关。因此，FBP结合位点高表达的34例患者中有15例发生了淋巴结转移，而FBP结合位点低表达或无表达的17例患者未发生淋巴结转移。所有转移淋巴结均与FBP发生强烈反应。此外，与pTa、pT1或pT2期的原发性病变相比，pT3b或pT4期的原发性病变与FBP的反应更为频繁。虽然已知FBP能与Galβ1----4（Fucα1----3）GlcNAc和Fucα1----2Gal序列发生反应，但对其他碳水化合物标记物的对比染色显示，后一种结构与转移潜能和分期无关。
