Matsusako T, Muramatsu H, Shirahama T, Muramatsu T, Ohi Y
Department of Urology, Faculty of Medicine, Kagoshima University, Japan.
Histochem J. 1992 Nov;24(11):805-10. doi: 10.1007/BF01046352.
We have previously shown that the degree of expression of Lex-related carbohydrate epitopes, namely, Lotus tetragonolobus agglutinin (LTA) receptors, SSEA-1 and FH6, correlates with the metastatic potential of transitional cell carcinoma of the human urinary bladder. In an effort to obtain a better reagent with which to detect a metastasis-associated epitope, monoclonal antibodies were produced against LTA receptors from BOY bladder carcinoma cells. One antigen defined by such a monoclonal antibody, MM4, indeed showed better correlation with the metastatic potential of the tumour than did other carbohydrate markers. In the LTA receptors, MM4 antigen was located only on a 60 kDa glycoprotein. In extracts from primary carcinomas and lymph node metastases, the 60 kDa glycoprotein was the principal carrier of MM4 antigen. LTA receptors from these sources were composed of arrays of glycoproteins, while the 60 kDa one was invariably present. Metastasis-associated carbohydrate epitopes on the 60 kDa glycoprotein may promote metastasis by interaction with carbohydrate-recognizing proteins such as selectins on host cells.
我们之前已经表明，Lex相关碳水化合物表位（即百脉根凝集素（LTA）受体、阶段特异性胚胎抗原-1（SSEA-1）和FH6）的表达程度与人膀胱移行细胞癌的转移潜能相关。为了获得一种更好的试剂来检测转移相关表位，我们制备了针对BOY膀胱癌细胞LTA受体的单克隆抗体。由这种单克隆抗体（MM4）定义的一种抗原，确实比其他碳水化合物标志物与肿瘤的转移潜能表现出更好的相关性。在LTA受体中，MM4抗原仅位于一种60 kDa的糖蛋白上。在原发性癌和淋巴结转移灶的提取物中，60 kDa糖蛋白是MM4抗原的主要载体。这些来源的LTA受体由糖蛋白阵列组成，而60 kDa的糖蛋白始终存在。60 kDa糖蛋白上的转移相关碳水化合物表位可能通过与宿主细胞上的碳水化合物识别蛋白（如选择素）相互作用来促进转移。
