Norton R S, Macek P, Reid G E, Simpson R J
School of Biochemistry, University of New South Wales, Kensington, Australia.
Toxicon. 1992 Jan;30(1):13-23. doi: 10.1016/0041-0101(92)90497-s.
The chemical, physical and biological properties of the cytolysin tenebrosin-C from Actinia tenebrosa have been compared with those of equinatoxin II from Actinia equina. The two proteins are indistinguishable by reverse-phase and cation-exchange HPLC and capillary zone electrophoresis, and give similar peptide fragments upon cyanogen bromide cleavage (as judged by the chromatographic behaviour, ultraviolet absorption spectra, amino acid composition and N-terminal amino acid sequences of the peptides). Their cardiac stimulatory activities are identical, and their haemolytic activities are similar, with equinatoxin II having slightly greater activity. These data indicate that the two molecules are either identical in all 179 amino acid positions, or differ by no more than one or two residues. These findings are discussed in the context of the taxonomic relationship between the two species of sea anemone.
已将来自壮丽海葵的细胞溶素——暗黑菌素-C的化学、物理和生物学特性与来自马氏海葵的海葵毒素II的特性进行了比较。通过反相和阳离子交换高效液相色谱法以及毛细管区带电泳，这两种蛋白质无法区分，并且在溴化氰裂解后产生相似的肽片段（根据肽的色谱行为、紫外吸收光谱、氨基酸组成和N端氨基酸序列判断）。它们的心脏刺激活性相同，溶血活性相似，海葵毒素II的活性略高。这些数据表明，这两个分子在所有179个氨基酸位置上要么完全相同，要么差异不超过一两个残基。将结合这两种海葵的分类学关系来讨论这些发现。
