Komatsu S, Furukawa K, Abe K, Hirano H, Ueda M
Department of Molecular Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
Chem Pharm Bull (Tokyo). 1992 Oct;40(10):2873-5. doi: 10.1248/cpb.40.2873.
Equinatoxins were purified from the tentacles and bodies of the sea anemone Actinia equina by the use of acetone precipitation, as well as column chromatographies on Sephadex G-50 and CM-cellulose according to the modified method of Macek and Lebez (1988). The equinatoxins obtained, equinatoxin 1 and 2, were hemolytic glycoproteins with a relative molecular mass of 20000. Equinatoxin 2 was found to be rich in glycine, alanine and valine. The amino acid sequences of equinatoxins 1 and 2 were partially determined. A portion of the N-terminal amino acid sequence of equinatoxin 2 was similar to those of pyruvate kinase and sialidase.
根据Macek和Lebez（1988年）的改良方法，通过丙酮沉淀以及在Sephadex G - 50和CM - 纤维素上的柱色谱法，从海葵黄海葵的触手和身体中纯化出了海葵毒素。所获得的海葵毒素，即海葵毒素1和2，是相对分子质量为20000的溶血糖蛋白。发现海葵毒素2富含甘氨酸、丙氨酸和缬氨酸。部分测定了海葵毒素1和2的氨基酸序列。海葵毒素2的一部分N端氨基酸序列与丙酮酸激酶和唾液酸酶的序列相似。
