Thomson M, Moritz R L, Simpson R J, Norton R S
School of Biochemistry, University of New South Wales, Kensington, Australia.
Biochem Int. 1987 Oct;15(4):711-8.
A new cardiac stimulatory protein, tenebrosin-A, has been isolated from the Australian sea anemone Actinia tenebrosa by gel filtration and cation-exchange chromatography, followed by cation-exchange HPLC. Its purity is established by analytical reversed-phase HPLC and N-terminal sequence analysis. According to SDS-PAGE, its apparent Mr is 20,000 daltons. Amino acid analysis indicates that it contains 186 residues, and is devoid of cysteine or cystine. Tenebrosin-A exerts a strong positive inotropic effect on isolated guinea pig atria at a concentration of 1.4 nM, with little chronotropic activity.
一种新的心脏刺激蛋白——暗黑菌素-A，已通过凝胶过滤和阳离子交换色谱法，随后进行阳离子交换高效液相色谱法，从澳大利亚海葵暗黑海葵中分离出来。其纯度通过分析型反相高效液相色谱法和N端序列分析得以确定。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，其表观分子量为20,000道尔顿。氨基酸分析表明它含有186个残基，且不含半胱氨酸或胱氨酸。暗黑菌素-A在浓度为1.4纳摩尔时，对分离的豚鼠心房产生强烈的正性肌力作用，而变时活性很小。
