Yamanaka K, Takasaki Y, Nishida Y, Shimada K, Shibata M, Hashimoto H
Department of Internal Medicine, Juntendo University, School of Medicine, Tokyo, Japan.
Arthritis Rheum. 1992 Jun;35(6):667-71. doi: 10.1002/art.1780350610.
To establish an enzyme-linked immunosorbent assay (ELISA) for detecting anti-Ki antibody, using a bovine recombinant Ki antigen, and studying its specificity.
Sera from 220 patients with various connective tissue diseases were screened, and a prospective study of fluctuations in anti-Ki antibody and clinical course of a woman with systemic lupus erythematosus (SLE) was analyzed, by ELISA:
Anti-Ki antibodies were present in 18.9% of patients with SLE. The titer of anti-Ki antibody in the woman with SLE rose before the onset of pericarditis and pleuritis in this longitudinal study.
ELISA using a recombinant Ki antigen is useful for the diagnosis of SLE, and it might be useful in estimating disease activity in patients with SLE.
利用牛重组Ki抗原建立一种检测抗Ki抗体的酶联免疫吸附测定（ELISA）法，并研究其特异性。
对220例各种结缔组织病患者的血清进行筛查，并通过ELISA对一名系统性红斑狼疮（SLE）女性患者的抗Ki抗体波动情况及其临床病程进行前瞻性研究。
18.9%的SLE患者存在抗Ki抗体。在这项纵向研究中，该SLE女性患者的心包炎和胸膜炎发作前，其抗Ki抗体滴度升高。
使用重组Ki抗原的ELISA法对SLE的诊断有用，且可能有助于评估SLE患者的疾病活动度。
