Sakamoto M, Takasaki Y, Yamanaka K, Kodama A, Hashimoto H, Hirose S
Department of Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Arthritis Rheum. 1989 Dec;32(12):1554-62. doi: 10.1002/anr.1780321209.
Ki antigen from rabbit thymus extract was purified by ammonium sulfate precipitation, anti-Ki affinity chromatography, and high pressure liquid chromatography gel filtration. The purified Ki antigen gave a single polypeptide with an MW of 32,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it specifically reacted with anti-Ki antibody by immunoblotting. The isoelectric point of the Ki antigen was found by isoelectric focusing to be 4.3. An enzyme-linked immunosorbent assay using the purified Ki antigen was established, and the clinical significance of the anti-Ki antibody in systemic lupus erythematosus (SLE) was studied. Thirty of 140 patients with SLE (21.4%) had anti-Ki antibody by enzyme-linked immunosorbent assay, whereas 11 (7.9%) were positive by double immunodiffusion. Analysis of clinical and serologic data on patients with SLE suggested a higher prevalence of central nervous system involvement in patients with anti-Ki antibody.
通过硫酸铵沉淀、抗Ki亲和层析和高压液相色谱凝胶过滤法对兔胸腺提取物中的Ki抗原进行了纯化。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，纯化后的Ki抗原呈现出一条分子量为32,000的单一多肽带，并且通过免疫印迹法可与抗Ki抗体发生特异性反应。通过等电聚焦法测定Ki抗原的等电点为4.3。利用纯化后的Ki抗原建立了酶联免疫吸附测定法，并对系统性红斑狼疮（SLE）患者体内抗Ki抗体的临床意义进行了研究。在140例SLE患者中，有30例（21.4%）通过酶联免疫吸附测定法检测出抗Ki抗体，而通过双向免疫扩散法检测出阳性的有11例（7.9%）。对SLE患者的临床和血清学数据进行分析表明，抗Ki抗体阳性的患者中枢神经系统受累的发生率更高。
