Taylor-Robinson D, Gilroy C B, Thomas B J, Keat A C
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, UK.
Lancet. 1992 Jul 11;340(8811):81-2. doi: 10.1016/0140-6736(92)90399-n.
In 1986, Chlamydia trachomatis elementary bodies were found by direct immunofluorescence (DIF) in synovial-fluid cell deposits and synovial-membrane biopsy samples from five of eight patients with sexually acquired reactive arthritis (SARA) but in none of eight controls with other types of arthritis. Cells from the original slides (stored at 4 degrees C) have now been examined by a polymerase chain reaction (PCR) that amplifies DNA for the major outer membrane protein of C trachomatis. Chlamydial DNA was found in samples from four DIF-positive patients, one DIF-negative patient, and one DIF-negative control. Overall, there was 80% concordance for DIF and PCR results. This study supports our previous finding of chlamydiae in joints in reactive arthritis.
1986年，通过直接免疫荧光法（DIF）在8例性传播感染反应性关节炎（SARA）患者中的5例的滑液细胞沉积物和滑膜活检样本中发现了沙眼衣原体原体，但在8例其他类型关节炎的对照患者中均未发现。现已通过聚合酶链反应（PCR）对原始载玻片（保存在4摄氏度）上的细胞进行检测，该反应可扩增沙眼衣原体主要外膜蛋白的DNA。在4例DIF阳性患者、1例DIF阴性患者和1例DIF阴性对照的样本中发现了衣原体DNA。总体而言，DIF和PCR结果的一致性为80%。本研究支持了我们之前在反应性关节炎关节中发现衣原体的结果。
