Poole E S, Highton J, Wilkins R J, Lamont I L
Pathology Service, Dunedin Hospital, New Zealand.
Br J Rheumatol. 1992 Jan;31(1):31-4. doi: 10.1093/rheumatology/31.1.31.
A polymerase chain reaction (PCR) technique to detect Chlamydia trachomatis DNA was used to examine synovial specimens from patients with reactive arthritis. We were able to detect C. trachomatis DNA in synovial specimens which had been seeded with intact elementary bodies or chlamydial DNA. However, we were unable to detect chlamydial DNA in unseeded synovial specimens from 10 patients with sexually acquired reactive arthritis, 17 patients with reactive arthritis and 11 control patients with other arthropathies. In addition, using a monoclonal antibody technique, we were unable to detect chlamydial antigen in any of the synovial cell deposits examined. We conclude that C. trachomatis DNA was not present in the joints of these patients at the time of synovial fluid collection, and suggest that either DNA degradation occurred rapidly after viable chlamydiae had entered the joint or that chlamydial DNA was not present at any stage of the reactive response.
采用聚合酶链反应（PCR）技术检测沙眼衣原体DNA，以此检查反应性关节炎患者的滑膜标本。我们能够在接种了完整原体或衣原体DNA的滑膜标本中检测到沙眼衣原体DNA。然而，我们未能在10例性传播获得性反应性关节炎患者、17例反应性关节炎患者以及11例患有其他关节病的对照患者的未接种滑膜标本中检测到衣原体DNA。此外，运用单克隆抗体技术，我们在所检查的任何滑膜细胞沉积物中均未检测到衣原体抗原。我们得出结论，在收集滑液时，这些患者的关节中不存在沙眼衣原体DNA，并推测要么是活衣原体进入关节后DNA迅速降解，要么是在反应性应答的任何阶段都不存在衣原体DNA。
