在培养的人T84肠上皮层中，由P-糖蛋白介导的容积激活的氯离子分泌与跨上皮长春碱分泌不相关。

Volume-activated Cl- secretion and transepithelial vinblastine secretion mediated by P-glycoprotein are not correlated in cultured human T84 intestinal epithelial layers.

作者信息

McEwan G T, Hunter J, Hirst B H, Simmons N L

机构信息

Gastrointestinal Drug Delivery Research Centre, University of Newcastle upon Tyne, Medical School, UK.

出版信息

FEBS Lett. 1992 Jun 15;304(2-3):233-6. doi: 10.1016/0014-5793(92)80626-r.

DOI:10.1016/0014-5793(92)80626-r

PMID:1352259

Abstract

The relationship between the P-glycoprotein-mediated vinblastine secretion and cell-swelling activated Cl- secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 microM 1,9-dideoxy-forskolin, volume-stimulated Cl- secretion is unaffected. In contrast, 100 microM 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS) inhibited the volume-stimulated Cl- secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume-stimulated Cl- secretion but maintained a normal level of secretory vinblastine flux.

摘要

研究了人结肠腺癌T84细胞完整上皮层中P-糖蛋白介导的长春碱分泌与细胞肿胀激活的Cl⁻分泌（电导）之间的关系。虽然100μM 1,9-二脱氧佛司可林可有效抑制长春碱分泌，但容积刺激的Cl⁻分泌不受影响。相反，100μM 4,4'-二异硫氰酸芪-2,2'-二磺酸（DIDS）抑制了容积刺激的Cl⁻分泌，但对跨上皮长春碱分泌没有影响。此外，还注意到一些上皮层未能表达容积刺激的Cl⁻分泌，但分泌性长春碱通量保持正常水平。