Bershadskiĭ A D, Ivanova O Iu, Liass L A, Pletiushkina O Iu, Vasil'ev Iu M, Gel'fand I M
Tsitologiia. 1992;34(2):18-22.
A tumor promoter phorbol 12-myristate 13-acetate (PMA) induces characteristic reversible changes in the cell shape in certain fibroblastic lines. This reaction to PMA may be regarded as a prototype of reorganizations involving formation of stable cytoplasmic processes. Two specific drugs, Taxol and Colcemid, were used to study the role of microtubules and vimentin-containing intermediate filaments (IF) in the development of PMA-induced reorganizations. A short (I h) exposure to PMA induced formation of processes in the control cells rather than in the Colcemid treated cells having depolymerized microtubules and the IF that collapsed around the nucleus. A longer (3-4 h) exposure to PMA of the colcemid-treated cells induced a partial reversal of the IF collapse; those parts of peripheral lamellae that contained IF were transformed into narrow noncontractile processes. It is suggested that the local interaction of the IF with the actin system is an essential step in the formation of processes from lamellae.
肿瘤促进剂佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）可诱导某些成纤维细胞系细胞形态发生特征性的可逆变化。这种对PMA的反应可被视为涉及稳定细胞质突起形成的重组的一个原型。使用两种特定药物紫杉醇和秋水仙酰胺，来研究微管和含波形蛋白的中间丝（IF）在PMA诱导的重组发展过程中的作用。短时间（1小时）暴露于PMA会在对照细胞中诱导突起形成，而在微管已解聚且IF在细胞核周围塌陷的秋水仙酰胺处理的细胞中则不会。对秋水仙酰胺处理的细胞长时间（3 - 4小时）暴露于PMA会诱导IF塌陷的部分逆转；外周薄片中含有IF的那些部分会转变为狭窄的非收缩性突起。有人提出，IF与肌动蛋白系统的局部相互作用是薄片形成突起过程中的一个关键步骤。
