Wu J, Zhu J Q, Zhu D X, Scharfman A, Lamblin G, Han K K
Department of Biochemistry, Nanjing University, People's Republic of China.
Cell Mol Biol. 1992 Jul;38(4):367-76.
Multiple homeobox genes are expressed in haematopoietic cell lineages and their expression is cell-type specific. Thus we hypothesized that certain homeobox genes may play an important role in the process of haematopoiesis. To prove that issue, normal murine bone marrow cells were stimulated with appropriate Colony Stimulating Factors in the presence of mouse homeobox gene (Hox 2.3) sense or antisense oligodeoxynucleotides and the effects on the haematopoietic colony formation were examined. Treatment of the cells to Hox 2.3 antisense oligodeoxynucleotides led to a selective inhibition of myeloid colony formation, both in size and in numbers, but without significant effect on erythroid and megakaryocytic haematopoiesis. Exposure to Hox 2.3 sense oligodeoxynucleotides (no-oligomers), had no such effect. It was further showed that inhibition of myelopoiesis by Hox 2.3 antisense oligodeoxynucleotides was dependent on the differentiation stage of target cells. These findings demonstrated that Hox 2.3 gene plays a critical role in regulating normal murine myelopoiesis.
多个同源框基因在造血细胞谱系中表达，且其表达具有细胞类型特异性。因此，我们推测某些同源框基因可能在造血过程中发挥重要作用。为了证实这一问题，在小鼠同源框基因（Hox 2.3）正义或反义寡脱氧核苷酸存在的情况下，用适当的集落刺激因子刺激正常小鼠骨髓细胞，并检测其对造血集落形成的影响。用Hox 2.3反义寡脱氧核苷酸处理细胞会导致髓系集落在大小和数量上均受到选择性抑制，但对红系和巨核系造血没有显著影响。暴露于Hox 2.3正义寡脱氧核苷酸（无寡聚物）则没有这种效果。进一步表明，Hox 2.3反义寡脱氧核苷酸对骨髓生成的抑制作用取决于靶细胞的分化阶段。这些发现表明，Hox 2.3基因在调节正常小鼠骨髓生成中起关键作用。
